Instructions

Tips

For best results and turnaround time:

  • Live, healthy, mitotically-active cells in log phase of growth yield the best cytogenetic starting materials.
  • Flasks of adherent cultures should be subconfluent, minimizing contact inhibition of cells. We recommend sending two 25cm2 flasks at approximately 25% and 50% confluency for faster growing cells, and three flasks at 25%, 50%, and 75% for slower growing cells.
  • Send at least two 25cm2 flasks per adherent cell line. We realize this is not always possible, however, this allows us to have a backup in the case one breaks during shipment, and also helps us to process cells under several different conditions and use what works best.
  • Label everything clearly.
  • If your cells grow best with daily changes of media (e.g. stem cells), please include an aliquot of complete media sufficient for two days of culture.
  • When shipping, send cells by overnight transport so they spend as little time outside an incubator as possible.
  • When shipping, do not include any cold packs or ice packs! This way cells will continue proliferating, albeit slowly, during transport.
  • When shipping, place flasks of adherent cultures right side up in their box (with respect to the shipping label) and indicate "UP" on the outside of the box so gravity will help cells remain attached.
  • For all orders, submit your Order Form at least 24 hours before you expect to have your samples dropped off, picked up, or delivered.

For local clients at UNC-Chapel Hill, Chapel Hill, NC

Clients at UNC-Chapel Hill may opt to:
Drop off specimens at the UNC Lineberger Comprehensive Cancer Center Tissue Culture Facility
OR
Have a KaryoLogic courier come to your lab
OR
Drop off specimens at KaryoLogic, Inc. at 5501 Fortunes Ridge Drive, Suite O, Durham, NC 27713.

  • For adherent cell lines, prepare two or three 25cm2 flasks with cells at varying confluencies, (e.g. 25%, 50%, and 75%) with a minimum of 500,000 cells seeded per flask, for each cell line.
  • For suspension cultures, place at least 10 ml of the cell culture in a 15 ml tube, and also include a tube of 10 ml complete media.
  • For stem cells typically grown on plates, prepare at least four subconfluent wells of a 6-well plate coated with basement membrane, and include a tube of 10 ml of complete media.
  • Cells on feeder layers are not a problem.
  • Label everything clearly.
  • Place your order "here" preferably at least 24 hours in advance of your estimated drop off or pick up time.

If you choose to work through UNC’s Tissue Culture Facility, bring flasks to the third floor of the Lineberger Comprehensive Cancer Center, Room 318, Monday through Friday, 8:00 AM to noon, or 1:00 PM to 5:30 PM. Billing will be through the Tissue Culture Facility system.

Alternatively, someone from KaryoLogic will contact you to arrange the details of when and where to pick up samples from your UNC Chapel Hill location and billing will be through your laboratory’s individual department accounting office.

Another option is to drop off specimens at KaryoLogic, Inc. at 5501 Fortunes Ridge Drive, Suite O, Durham, NC 27713.

For local clients at other locations in Durham, Wake, and Orange County, NC

  • For adherent cell lines, prepare two or three 25cm2 flasks with cells at varying confluencies, (e.g. 25%, 50%, and 75%) with a minimum of 500,000 cells seeded per flask, for each cell line.
  • For suspension cultures, place at least 10 ml of the cell culture in a 15 ml tube, and also include a tube of 10 ml complete media.
  • For stem cells typically grown on plates, prepare at least 4 wells of a 6-well plate coated with basement membrane, and include a tube of 10 ml of complete media.
  • Cells on feeder layers are not a problem.
  • Label everything clearly.
  • Place your order "here" preferably at least 24 hours in advance of your estimated drop off or pick up time.
    KaryoLogic will contact you to arrange the details of when and where to pick up your samples. Alternatively, specimens can be dropped off at KaryoLogic’s Durham location.

For long distance clients:
To prepare cells for shipping to KaryoLogic, Inc.
5501 Fortunes Ridge Drive, Suite O
Durham, NC 27713

  • For adherent cell lines, prepare two or three 25cm2 flasks with cells at varying confluencies, (e.g. 25%, 50%, and 75%) with a minimum of 500,000 cells seeded per flask, for each cell line.
  • For stem cells grown on basement membrane, coat the surfaces of 25cm2 flasks as you would plates. Seed cell aggregates to at least 20% confluency. Include a tube of at least 10 ml of complete media. Sending one flask is fine, though two is optimal.
  • Fill each flask to the top with complete media.
  • Tighten the caps and wrap well with parafilm around the necks and caps of the flasks. (Vented caps are fine.)
  • For suspension cultures, place at least 10 ml of the cell culture (minimum 500,000 cells) in a 15 ml tube, and wrap the top with parafilm. Also include a tube of 10 ml complete media.
  • DO NOT include cold packs or ice packs.
  • Cells on feeder layers are not a problem.
  • Label everything clearly. Enclose any special instructions.
  • Place flasks of adherent cultures right side up in their shipping container (with respect to the shipping label) to help cells remain attached.
  • Triple package. For example, place inside a plastic bag, then Styrofoam container, then cardboard box. Fill the empty spaces with paper towels or packing material.
  • Ship overnight at ambient temperature to:
    KaryoLogic, Inc.
    5501 Fortunes Ridge Drive, Suite O
    Durham, NC 27713
  • Place your order "here" preferably at least 24 hours in advance of the estimated arrival.

    For more information on shipping live cells see http://images.fedex.com/downloads/shared/packagingtips/pointers.pdf for Federal Express.

    For UPS, see http://www.ups.com/content/us/en/resources/ship/hazardous/biological_substances.html.